Supplementary resources¶

Educational content¶

The following resources are useful for learning more about microbiome analysis and bioinformatics:

• An Introduction to Applied Bioinformatics

• Gut Check: Exploring Your Microbiome

• Guide to Statistical Analysis in Microbial Ecology

Sample preparation protocols¶

• Primers and amplification protocols for 454 FLX+ long read 16S and 18S amplicons (version 13_4)

• 16S/18S/ITS Extraction, PCR, and Illumina HiSeq/MiSeq Protocols: Earth Microbiome Project Protocols Site

• 16S Illumina GAIIx Protocols (these differ from those for the HiSeq/MiSeq): Caporaso et al, 2010