Supplementary resources¶
Warning
This site has been replaced by the new QIIME 2 “amplicon distribution” documentation, as of the 2025.4 release of QIIME 2. You can still access the content from the “old docs” here for the QIIME 2 2024.10 and earlier releases, but we recommend that you transition to the new documentation at https://amplicon-docs.qiime2.org. Content on this site is no longer updated and may be out of date.
Are you looking for:
the QIIME 2 homepage? That’s https://qiime2.org.
learning resources for microbiome marker gene (i.e., amplicon) analysis? See the QIIME 2 amplicon distribution documentation.
learning resources for microbiome metagenome analysis? See the MOSHPIT documentation.
installation instructions, plugins, books, videos, workshops, or resources? See the QIIME 2 Library.
general help? See the QIIME 2 Forum.
Old content beyond this point… 👴👵
Educational content¶
The following resources are useful for learning more about microbiome analysis and bioinformatics:
Sample preparation protocols¶
Primers and amplification protocols for 454 FLX+ long read 16S and 18S amplicons (version 13_4)
16S/18S/ITS Extraction, PCR, and Illumina HiSeq/MiSeq Protocols: Earth Microbiome Project Protocols Site
16S Illumina GAIIx Protocols (these differ from those for the HiSeq/MiSeq): Caporaso et al, 2010