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dereplicate-sequences: Dereplicate sequences.¶
Docstring:
Usage: qiime vsearch dereplicate-sequences [OPTIONS] Dereplicate sequence data and create a feature table and feature representative sequences. Feature identifiers in the resulting artifacts will be the sha1 hash of the sequence defining each feature. If clustering of features into OTUs is desired, the resulting artifacts can be passed to the cluster_features_* methods in this plugin. Inputs: --i-sequences ARTIFACT SampleData[Sequences] | SampleData[SequencesWithQuality] | SampleData[JoinedSequencesWithQuality] The sequences to be dereplicated. [required] Parameters: --p-derep-prefix / --p-no-derep-prefix Merge sequences with identical prefixes. If a sequence is identical to the prefix of two or more longer sequences, it is clustered with the shortest of them. If they are equally long, it is clustered with the most abundant. [default: False] --p-min-seq-length INTEGER Range(1, None) Discard sequences shorter than this integer. [default: 1] --p-min-unique-size INTEGER Range(1, None) Discard sequences with a post-dereplication abundance value smaller than integer. [default: 1] Outputs: --o-dereplicated-table ARTIFACT FeatureTable[Frequency] The table of dereplicated sequences. [required] --o-dereplicated-sequences ARTIFACT FeatureData[Sequence] The dereplicated sequences. [required] Miscellaneous: --output-dir PATH Output unspecified results to a directory --verbose / --quiet Display verbose output to stdout and/or stderr during execution of this action. Or silence output if execution is successful (silence is golden). --example-data PATH Write example data and exit. --citations Show citations and exit. --use-cache DIRECTORY Specify the cache to be used for the intermediate work of this action. If not provided, the default cache under $TMP/qiime2/will be used. IMPORTANT FOR HPC USERS: If you are on an HPC system and are using parallel execution it is important to set this to a location that is globally accessible to all nodes in the cluster. --help Show this message and exit.
Import:
from qiime2.plugins.vsearch.methods import dereplicate_sequences
Docstring:
Dereplicate sequences. Dereplicate sequence data and create a feature table and feature representative sequences. Feature identifiers in the resulting artifacts will be the sha1 hash of the sequence defining each feature. If clustering of features into OTUs is desired, the resulting artifacts can be passed to the cluster_features_* methods in this plugin. Parameters ---------- sequences : SampleData[Sequences] | SampleData[SequencesWithQuality] | SampleData[JoinedSequencesWithQuality] The sequences to be dereplicated. derep_prefix : Bool, optional Merge sequences with identical prefixes. If a sequence is identical to the prefix of two or more longer sequences, it is clustered with the shortest of them. If they are equally long, it is clustered with the most abundant. min_seq_length : Int % Range(1, None), optional Discard sequences shorter than this integer. min_unique_size : Int % Range(1, None), optional Discard sequences with a post-dereplication abundance value smaller than integer. Returns ------- dereplicated_table : FeatureTable[Frequency] The table of dereplicated sequences. dereplicated_sequences : FeatureData[Sequence] The dereplicated sequences.