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decontam-identify: Identify contaminants¶
Docstring:
Usage: qiime quality-control decontam-identify [OPTIONS] This method identifies contaminant sequences from an OTU or ASV table and reports them to the user Inputs: --i-table ARTIFACT FeatureTable[Frequency] Feature table which contaminate sequences will be identified from [required] Parameters: --m-metadata-file METADATA... (multiple metadata file indicating which samples in the arguments will be experiment are control samples, assumes sample names merged) in file correspond to the `table` input parameter [required] --p-method TEXT Choices('combined', 'frequency', 'prevalence') Select how to which method to id contaminants with; Prevalence: Utilizes control ASVs/OTUs to identify contaminants, Frequency: Utilizes sample concentration information to identify contaminants, Combined: Utilizes both Prevalence and Frequency methods when identifying contaminants [default: 'prevalence'] --p-freq-concentration-column TEXT Input column name that has concentration information for the samples [optional] --p-prev-control-column TEXT Input column name containing experimental or control sample metadata [optional] --p-prev-control-indicator TEXT indicate the control sample identifier (e.g. "control" or "blank") [optional] Outputs: --o-decontam-scores ARTIFACT FeatureData[DecontamScore] The resulting table of scores from the decontam algorithm which scores each feature on how likely they are to be a contaminant sequence [required] Miscellaneous: --output-dir PATH Output unspecified results to a directory --verbose / --quiet Display verbose output to stdout and/or stderr during execution of this action. Or silence output if execution is successful (silence is golden). --example-data PATH Write example data and exit. --citations Show citations and exit. --use-cache DIRECTORY Specify the cache to be used for the intermediate work of this action. If not provided, the default cache under $TMP/qiime2/will be used. IMPORTANT FOR HPC USERS: If you are on an HPC system and are using parallel execution it is important to set this to a location that is globally accessible to all nodes in the cluster. --help Show this message and exit.
Import:
from qiime2.plugins.quality_control.methods import decontam_identify
Docstring:
Identify contaminants This method identifies contaminant sequences from an OTU or ASV table and reports them to the user Parameters ---------- table : FeatureTable[Frequency] Feature table which contaminate sequences will be identified from metadata : Metadata metadata file indicating which samples in the experiment are control samples, assumes sample names in file correspond to the `table` input parameter method : Str % Choices('combined', 'frequency', 'prevalence'), optional Select how to which method to id contaminants with; Prevalence: Utilizes control ASVs/OTUs to identify contaminants, Frequency: Utilizes sample concentration information to identify contaminants, Combined: Utilizes both Prevalence and Frequency methods when identifying contaminants freq_concentration_column : Str, optional Input column name that has concentration information for the samples prev_control_column : Str, optional Input column name containing experimental or control sample metadata prev_control_indicator : Str, optional indicate the control sample identifier (e.g. "control" or "blank") Returns ------- decontam_scores : FeatureData[DecontamScore] The resulting table of scores from the decontam algorithm which scores each feature on how likely they are to be a contaminant sequence